Investigation of the effect of olive leaf extract on the instestinal incision wound healing in rats

The study realized that was aimed to investigate the effects of olive leaf extract on healing of intestinal incision wounds in rats. In the study was realized on 32 adult Wistar Albino male rats. The rats applied general anesthesia on the first daywith followed by median laparotomy, a 2-3 cm incision was made in the descendens colon region to create a wound. Subsequently, the surgical region was closed according to appropriate surgical procedures. Groups were divided into four group as a Control, Sham, Group 1 and, Group 2. After administering 200 mg/kg/day of olive leaf extract to Group 1 on the 3rd day (n=4) and 7th day (n=4), and 400 mg/kg/day of olive leaf extract to Group 2 on the 3rd day (n=4) and 7th day (n=4) via gastric gavage applied, after that rats were sacrificed and histopathological, biochemical, and hematological evaluations. As a result of the histopathological evaluations, statistically significant differences (p<0.05) were observed in the inflammatory cell, fibroblastic, neovascularization, and collagen values in Group 1 and Group 2, which applied olive leaf treatment on the 3rd and 7th days, compared to the Control and Sham groups. In the biochemical evaluations, statistically significant differences were observed (p<0.05) in the TAS (p* 0.010), TOS (p* 0.001), IL-1 (p*0.000), IL-6 (p*0.018), TNF-α (p*0.14), and NO (p*0.001) values, except for HYP (p* 0.693). Statistically significant differences were observed in the hematology values of GRA, GRA(%), and PLT (p<0.05). As a consequently; histopathological, biochemical and clinical findings showed that olive leaf extract given by gavage for 3 days and 7 days, at doses of 200 mg/kg/day and 400 mg/kg/day, had a more positive effect on intestinal incision wound healing in rats, on healing on the 7th day. It has been revealed by hematological examinations, but we believe that more detailed research is needed.