Determination of the antioxidant activity of Rhabdosciadium anatoly plant and it's protective effect of chlorogenic acid in rats that cyclophosphamide-induced oxidative stress

This study aimed at investigating the determination of antioxidant, antiradical activity, vitamin C, mineral (Mg, Ca, K, P, Na) and trace element (Mn, Zn, Sr, Cu, Ti, Fe, V, Cr, Co, Pb, Mo, Tl, Sn, Cd, As) levels of R. anatolyi plant flower, which is an endemic plant grown in the Eastern Anatolia Region of our country, Also, investigate the protective effects of R. anatolyi plant flower ethanol extract and chlorogenic acid in rats that generate oxidative stress with cyclophosphamide (CP). The scope of the studied mineral and trace element analysis AAS and ICP-OES, vitamin C, R.anatolyi plant flower methanol extract total phenol, flavonoid, and antioxidant capacity, hydrogen peroxide scavenging activity, DPPH, superoxide, hydroxyl, ABTS, and radical scavenging in the phenylhydrazine induced hemolysis of erythrocytes activity of R.anatolyi plant flower was determined spectrophotometrically. At the experimental stage using 48 Wistar albino female rats 6 groups were assigned. Groups control group shaped % 0,9 NaCI, 200 mg kg-1 cyclophosphamide (CP) group, 100 mg kg-1 chlorogenic acid (CA) group, 300 mg kg-1 R. anatolyi (RA) group, 200 mg kg-1 CP + 300 mg kg-1 RA group and 200 mg kg-1 CP + 100 mg kg-1 CA group. The experimental stage was continued for 7 days and during this time plant extract and chlorogenic acid doses were given by gavages according to body weights of the rats. Oxidative stress (200 mg kg-1, intraperitoneal) provided with cyclophosphamide (CP). Rat's liver and kidney tissue samples were evaluated as an indicator of antioxidant properties CAT, SOD, GSH-Px specific enzymes and GSH, MDA, PCO, TSA, and LSA parameters were measured spectrophotometrically. Also in serum mineral (Ca, Mg, K, P, Na) and trace element (As, Cd, Co, Cr, Cu, Fe, Mn, Mo, Se, Sn, Sr, Ti, V, Zn) levels in liver and kidney tissue were evaluated as histopathological and immunohistochemical parameters. According to the results obtained in the liver and kidney tissues of rats 200 mg kg-1 CP administration in CAT, SOD and GSH-Px specific enzymes 200 mg kg-1 CP there was a statistically significant decreased (p <0.05) in the group applied compared to the control group. In the liver and kidney tissues MDA and PCO levels statistically significant increase (p <0.05, p <0.01, p <0.001) observed, At GSH levels 200 mg kg-1 CP was among other groups applied statistically a significant reduction (p <0.05, p <0.01, p <0.001) was detected. In both liver and kidney tissues TAS and LSA values considering the control group of 200 mg kg-1 CP applied group, between the group receiving 100 mg kg-1 CA and the group receiving 300 mg kg-1 RA statistically (p <0.001) significant increase was found. As a result of statistical analysis of serum mineral (Ca, K, Mg, Na) and trace elements (Co, Cu, Fe, Se, V, Zn) between the control group and the group receiving 200 mg kg-1 CP statistically decrease significant (p <0.05, p <0.01, p <0.001) were detected. As a result, the mineral, trace element, vitamin C, total phenol and flavonoid levels of R.anatolyi plant flower are high, the plant flower methanol extract was found to be effective in inhibiting free radicals. The results of histopathological and immunohistochemical findings were determined to be compatible with the biochemical parameters GSH-Px, SOD, CAT, MDA, GSH, PCO, TSA, and LSA levels. In liver and kidney histopathological and immunohistochemical examinations, 300 mg kg-1 R. anatolyi plant flower extract dose has a more pronounced effect compared to the dose of 100 mg kg-1 chlorogenic acid and this positive effect due to R. anatolyi plant flower is thought to be protective. Moreover, as a result of biochemical, histopathological, and immunohistochemical parameters in our study, it has been presented as a preliminary study of the literature.