A study on bioethanol production from roseau (Miscanthus giganteus) and cassava peels (Manihot esculenta) by Saccharomyces cerevisiae

In this study, bioethanol production from Miscanthus giganteus and cassava peel feedstocks was investigated. In order to convert hemicellulosic content to fermentable sugars miscanthus and cassava peels were treated at two different temperatures (120 and 180°C) for 10 and 90 min using NaOH ve H2SO4 at two different concentrations (0.5 ve % 1 (w/v)). The hydrolysates obtained after enzymatic hydrolysis with cellulases (Cellic CTec2 ) and amylolytic enzymes were fermented with Saccharomyces cerevisiae. Starch content of cassava peels was hydrolysed using -amylase and glucoamylase. Effect of temperature (25 and 30°C) and nitrogen supplementation (with pepton additon (4g.L-1) and without) on fermentation was also investigated. The pH of the medium was adjusted to 5.5. The maximum fermentable sugar obtained in the pyrolysis of miscanthus samples was 32.78g/L at 120°C for 90 min using % 0.5 (w/v) NaOH, whereas that for cassava peel was 30.12g/L at 120°C for 10 min using %1 (v/v) H2SO4. The maximum ethanol obtained during fermentration of miscanthus and cassaava peel hydrolysates were 12.59 ± 0.03 g/L ve 14.07 ± 0.63 g/L, respectively at 30°C with peptone supplementation.