The effects of treadmill exercise with energy drink supplementation on free radicals, antioxidants, angtpl8, elabela and lipid metabolism in rats

In our study; the aim of this study was to investigate the effects of treadmill exercise with energy drink supplementation on free radicals, antioxidants, Angtpl8, Elabela and lipid metabolism in rats. A total of 28 male rats 101,96 ± 9,75 grams were included in the study. Before the study, rats divided four groups as the control group (n = 7) given standard feed without any treatment; exercise group (n = 7) treadmill exercise performing at 30 cm/sc/speed with 0% incline on the treadmill for five days for four weeks; supplementation group (n = 7) given an energy drink by a single dose gavage daily (3.57 ml / kg / day) for four weeks; exercise + supplementation group (n = 7) given a single dose (3.57 ml / kg / day) energy drink by gavage and 30 minutes /day treadmill exercise was performed for four weeks. blood samples of the rats were obtained by decapitation. Angpt18, Ghrelin, Leptin, Irisin, Sod, Cat, Tbars, Tos, Taos parameters were carried out by ELISA method. Triglyceride, Cholesterol, HDL and LDL levels were analyzed by spectrophotometric method in autoanalyser. SPSS 22.0 package program was used in the analysis of the data. Since data were not normally distributed, Kruskal Wallis test was used. As a result of statistical analysis; statistically significant was found in Tbars (pg/mL), LDL (mg/dL), Irisin (pg/ml), Angpt18 (pg/ ml) and Elabela (ng/L) values in favor of the exercise group; SOD (ng/L) and HDL (mg/dL) values in favor of supplementation+exercise group; Cholesterol (mg/dL) levels in favor of the supplementation group (p<0,05). Although there were variations between the groups at Leptin, Ghrelin and Cat levels, statistical significance was not found (p> 0,05). As a result of the study; it could be said that treadmill exercise is important in lipid metabolism and regulation, stimulation of peptide hormones and receptors; given the energy drinks without exercise or physical activity increases fat deposits and increased fat in vital organs and tissues poses a threat to the body.