The Effect of Concurrent Thymoquin and Serum Application on WoundHealing in Rats

Acute wound development is caused by both physical and chemical elements that compromise the integrity of the skin. Because of its anti-inflammatory and antioxidant characteristics, thymoquinone aids in the healing of wounds. The healing effect of thymoquinone alone, in combination with serum, or neither was examined in this thesis study using an excisional wound model. The study employed male Wistar-Albino rats weighing 200–250 grams, aged 6–8 months. In our investigation, five groups were constituted (n = 8). In the upper back region of anesthetized rats, a full thickness 3 mm circular excision wound encompassing the epidermis, dermis, and hypodermis was generated. The experimental groups received once every 3 days thymoquinone, dimetyl sulfoxide, serum, and thymoquinone+serum solution for 21 days beginning on the first day following surgery, while the control group received no treatment. Following sacrification, the morphology and wound healing were assessed histologically. In histological assessments, Hematoxylin-Eosin and Masson trichrome stains were used. Thymoquinone application resulted in a statistically significant increase in epithelialization (p<0.05) in comparison to the control group and other groups, as well as a decrease in depth (P<0.05) in the thymoquinone group relative to the control group. Additionally, thymoquinone given in conjunction with serum was demonstrated to reduce wound depth in comparison to the control group (p=0.044 and p=0.02, respectively). Therefore, compared to the control group, it was noted that thymoquinone contributed to epithelialization when applied alone and with serum; however, the group that received serum application did not contribute as much to epithelialization as the group that received thymoquinone application alone (p<0.05). As a result, it was observed that thymoquinone contributed to epithelialization when applied alone and with serum compared to the control group; however, the contribution of thymoquinone application alone to epithelialization was found to be higher than the group applied with serum (p<0.05). In addition, thymoquinone applied alone and with serum reduced the wound depth compared to the control group and this decrease was found to be statistically significant (p=0.04 and p=0.02, respectively). However, statistically unsignificant difference was found between the wound healing between the two groups when thymoquinone was applied alone and with serum. Keywords: excisional wound model, wound healing, thymoquinone, serum