The Investigation of histological structure of tongue papillaries of pupy rat born form rat, sulajected to sialoadenectomy

SUMMARY THE INVESTIGATION OF HISTOLOGICAL STRUCTURE OF TONGUE PAPILLARIS OF PUPY RAT BORN FROM RATS SUBJECTED TO SIALOADENECTOMY The papillaries on the dorsal surface of tongue are constantly washed by saliva rich in Epidermal Growth Factor (EGF). After the complete removal of submandibular gland surgically (Sialoadenectomy), morphological alterations, and the effects of EGF given orally on these alterations were investigated. In the study, 30 adult female Spraque-Dawley rats were used, which were divided into three groups: Control group (n=10); sialoadenectomy group (SX, n= 10); sialoadenectomy + epidermal growth factor group (SX+EGF, n=10). Following a three week period of recovery, the rats were mated. On days 16- 19 of pregnancy, a total of 50 microgram (ug) EGF was given to the animals in SX + EGF group with orogastric tub as 1.25ug daily to each animal. The fetuses born following pregnancy were kept to grow up until the 28th day. The total weights of pupy rat of 28 days in all groups were taken, and then they were sacrificed. The paraffin section obtained through routine histological methods were stained with Hematoxylen-Eosine (H.E), Methylen blue- Basic fuchsin, Hemotoxylen-Van Gieson, and were examined under light microscope. Through an ocular micrometer, the measurements regarding papillary and taste buds were made. The results were statistically evaluated by one way variance analysis (ANOVA) and multicomparison test (TUKEY). We observed that, besides thickening and keratinization, substantial structural alterations in papillary and taste buds on the tongue epithelium of SX group. We also determined that EGF, given orally to SX+EGF group, ensured reversibly a remarkable improvement in those structural alterations in papillaries. Key Words: Rat, Epidermal Growth Factor, Sialoadenectomy, Lingual papillaries. vm