Effect of cigarette smoke exposure on rho/rho-kinase signalization pathway in mouse uterus

Cigarrette smoke exposure has side effects lungs, cardiovascular systems and some tissue and organs and most affected organs is lungs. Rho/rho-kinase signalization pathway has important role on uterus smooth muscle contractions. Mechanism of molecular of some side effects due to cigarette smoke exposure has not yet been clearly identified. In the present study we aimed to research effect of cigarette smoke exposure on rho/rho-kinase signalization pathway in uterus. For this propose mice (female age: 8 weeks) separated into two groups as control and smoke exposured. The cigarette smoke application continued 7 days in a week during two mouths. In the end of two mounts, Mice were killed by cervical dislocation and their uterus were isolated. Then rhoA and rho-kinase enzymes expression and rho-kinase enzyme activity have been determined. Cigarette smoke exposure unregulated rhoA and rho-Kinase enzyme expression and elevated rho-kinase enzyme activity. As a result cigarette smoke elevated activity of rho/rho-kinase signalization pathway.