The effect of the smoking on the oxidative stress and serum mineral element levels

SUMMARYIn this study, it is examined that the effect of the smoking on the oxidative stress andserum mineral element levels. With this aim, between 25 and 45 years old 25 men whohaven?t got any healty problem, who are not using a drug, vitamine, alcohol or same materialsexcept cigarette, smoking years between 5 or 10 years, daily smoking members changingbetween 15- 30 unit are the test grup of this study. 23 men who haven?t used cigarette oralcohol, in the same time who are not passive smokers at home or at work and changing theirages between 25 and 45 years olda re the control group of this study.Blood somples from control and test groups? members are taken for the aim of providingstandarization of the blood samples, these are taken after 12 hours hunger and on the sittinposition. Blood samples on 3000 rpm bye making centrifuge in ten minutes, serum andplasmas are seperated. On seperated serums iron (Fe) and magnesium (Mg) levels areexamined in the same day but serum samples fort he analysis of the copper (Cu) and zinc(Zn), by transferring eppendorf tubes in -20oC were hidden till the analysis day. From theplasmas that we have, ascorbic asid (Vit C) and malondialdehid ( MDA) measuring were donein the same time. For the superoxide dismutase (SOD) analysis, by preparing hemolysates oferythrocytes they were hidden till the analysis day in -20 oC degree.It is established that plasma MDA levels between non-smokers and smokers people oneby one as 6,14 ± 0,46 µmol/L and 11,99 ± 0,39 µmol/L, plasma Vit C levels are also one byone as 0,384 ± 0,062 mg/dL and 0,217 ± 0,027 mg/dL. In erythrocyte hemolysates SODenzyme activities were measured between non-smokers and smokers people one by one as30,96 ± 2,96 U/mgHgb and 39,19 ± 2,76 U/mgHgb. Serum copper levels were measuredbetween non-smokers and smokers people one by one as 49,48 ± 2,30 µg/dL ve 86,73 ± 5,05µg/d Land serum zinc levels were fixed between non-smokers people one by one as 27,45±0,77 µmol/L and 23,44 ± 0,62 µmol/L. Serum magnesium quantities were establishedbetween non-smokers and smokers people one by one as 2,12 ± 0,12 mg/dL ve 3,14 ± 0,53mg/dL. In the result of the evaluated findings, it was come to a conclusion that long timesmoking causes changes on antioxidane system and serum mineral substance level that withthis system and it will be useful to reinforcement on trace elements which are the cofactors ofthe antioxidane enzymes in the same time and ascorbic asid of the smoker diets.Key words: Cigarette, Oxidative stress, Antioxidane, Malondialdehyde, Superoxidedismutase, Ascorbic asid, trace elements