Optimisation of somatic embryogenesis in cyclamen (Cyclamen persicum)

In this study, the potential of somatic embryogenesis from different explants (ovule, divided ovary parts, leaf and petiole segments) of 15 plants from one species was studied. Explants were cultured in the medium containing ½ MS (macro and micro elements) and 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(g,g-dimethylallylamino) purine (2iP) for inducing embriyogenic callus. Results showed that there was a variation in terms of somatic embryogenessis potantial between explant types and plants. Although the best ratio of callus formation was distinguished on petiole, the best ratio of somatic embryo was distinguished on ovary. The ratios of callus formation were calculated on petiole, ovary, ovule and leaf %34.3, %30.16, %26.6 and %15.6 respectively. The ratios of somatic embryo production were calculated on ovary, petiole, leaf and ovule %11.3, %8.00, %4.16 and %2.83 respectively.