In vivo genotoxic effects of sodium metabisulfit on bone marrow cells

ABSTRACTPhD THESISIN VIVO GENOTOXIC EFFECTS OF SODIUM METABISULFIT ON BONEMARROW CELLSAhmet KAYRALDIZDEPARTMENT OF BIOLOGYINSTITUTE OF NATURAL AND APPLIED SCIENCESUNIVERSITY OF ÇUKUROVASupervisor : Prof. Dr. Mehmet TOPAKTAŞYear : 2005, Sayfa: 67Jury : Prof. Dr. Mehmet TOPAKTAŞProf. Dr. Rüştü HATİPOĞLUAssoc. Prof. Dr. Eyyüp RENCÜZOĞULLARIAssoc. Prof. Dr. Gökhan CORALAsist. Prof. Dr. Hasan Basri İLAThe aim of this study was to investigate the genotoxic effects of sodiummetabisulphite (SMB), which is used as an antimicrobial substance in foods on bone marrowcells of rats.In this study, SMB increased the percentages of abnormal cells with chromosomalabnormalities and CA/cell in all the concentrations (250, 500, 750 and 1000 mg/kg bodyweight (b.w.)) and treatment periods (6, 12 and 24 h) when compared with control afterintraperitonally administration. However, SMB did not increase the abnormallities as muchas positive control (Ethyl Carbamate). SMB decreased the Mitotic Index (MI) at 750 and1000 mg/kg b.w.concentrations for 6 hour treatment period while SMB decreased the MI inall the concentrations for 12 and 24 hours treatment periods when compared with control.SMB did not increase the percentage of abnormal cells and CA/cell in all theconcentrations for 6h treatment period when compared with control and positive control inbone marrow cells of rats treated with SMB orally (gavage). However, SMB significantlyincreased the abnormallities at all concentrations for 12 hour treatment period whencompared with control. At 24 hour treatment period, SMB increased the abnormalities onlyat 750 and 1000 mg/kg b.w. when compared with control. In all concentrations and treatmentperiods, SMB did not induced the abnormalities when compared with positive controls whenorally administreted. SMB decreased the MI at 1000 mg/kg b.w. for 6 h treatment periodwhen compared with positive control. At all concentrations for 12 h treatment period, SMBsignificantly decreased the MI when compared with control and positive control except 250mg/kg b.w. In addition, SMB significantly decreased the MI at all concentrations for 24 htreatment period according to control and positive control.Key Words: Food Additive, Sodium Metabisulphite, In vivo Chromosomal Aberrations, RatII