Effects of ozone therapy on the development of contralateral lung in rats that underwent left pneumonectomy

Objective: Pneumonectomy is one of the major surgeries of thoracic surgery and has become frequently used due to the increasing rate of lung cancers. Restricted respiration reserve after pneumonectomy is the most important factor that decreases patient?s life quality, extends hospital stay, time of returning back to daily activities and healthcare costs. Volume, weight, collagen content, protein and cellular size of contralateral lung increase after pneumonectomy. This situation is important for patients with preoperatively restricted respiratory reserve. Provision of size increase in residual lung shall contribute to improvement of respiratory functions. Few from many of the agents used for this purpose yielded positive results. In this study it is amied to investigate the effects of rectally administered preoperative and postoperative ozone therapy in rats to increase the development of the residual lung tissue.Material and Method: In this study, 21 adult, male Wistar albinor rats of the same colony were used. Rats were divided into three groups and the body weights were measured (A, B, C). Group A is the sham group and underwent only posterolateral left thoracotomy. Group B and C underwent left pneumonectomy after ligation of hilar structures with 4-0 silk-suture following posterolateral and left thoracotomy. C group is the only group that received ozone therapy every day for 5 days preoperatively in 10 µgr/ml, 0.36mg/kg doses and every day for 5 days postoperatively in 30 µgr/ml, 1.1mg/kg doses. Rats were sacrificed 7 days after surgery with ketamine of lethal doses and their total body weights were measured, right lung were extracted with trachea and the right lung weights were measured.. The pulmonary tissues were histologically examined. The lung volume and weight before sacrificing were proportioned with body weight and lung weight and volume indexes were found. The results were statistically assessed.