Effects of Zinc Supplementation on Lipid Peroxidation, and Lactate Levels in Rats with Streptozotocine-İnduced Diabetic and Acute Swimming Exercise.

SUMMARYThe aim of the this study was to investigate the effects of zinc supplementation on lipid peroxidation, antioxidant capacity and lactate levels in rats with streptozotocine-induced diabetic and acute swimming exercise.Research was performed on 80 male Spraque-Dawley strain rats in Selcuk University, Veterinary Faculty, Experiment Animals Unit. Study protocol was approved by ethics committee of Selcuk University School of Physical Education and Sport.Animals were divided into 8 groups including same number in all groups.Group 1 General Control Group: Animals were feeded by normal diet and no any application on animals.Group 2 Zinc Supplemented Control Group: Zinc sulphate were supplemented as 6 mg/kg/day by intraperitoneal in addition to normal diet.Group 3 Zinc Supplemented Diabetic Control Group: Animals were formed by subcutaneous Streptozotocin (STZ) ?40 mg/kg? and Zinc sulphate were supplemented as 6 mg/kg/day by intraperitoneal in addition to normal diet.Group 4 Swimming Control Group: Animals were feeded by normal diet and acute swimming exercise performed 30 minutes.Group 5 Zinc Supplemented Swimming Group: Animals were feeded by normal diet and zinc supplemented as 6 mg/kg/day by intra peritoneal for 4 weeks and swimming exercise for 30 minutes.Group 6 Zinc Supplemented Diabetic and Swimming Group: Firstly animals were performed diabetic by Streptozotocin (STZ) as 40 mg/kg, then zinc sulphate was supplemented as 6 mg/kg/day for 4 weeks and acute swimming exercise performed for 30 minutes.Group 7 Diabetic Swimming Group: Streptozotocin (STZ) ?40 mg/kg? applied by subcutaneous to induce diabetes and acute swimming exercise performed for 30 minutes.Group 8 Diabetic Group: Diabetic group which performed by subcutaneous Streptozotocin (STZ) as 40 mg/kg.Animals were decapited to obtain blood samples after the 4 weeks experimental period and by calorimetric method GSH in erythrocyte, GPX and SOD in serum, MDA and lactate in plasma were determined and serum zinc levels were determined by atomic emission.The highest MDA levels were obtain in group (p<0.001). MDA levels in group 8 were lower than group 7 and higher than all other groups (p<0.001). MDA levels of groups 3, 4 and 6 were lower than groups 7 and 8, but higher than groups 1, 2 and 5 (p<0.001).It has been determined that group 5 has the highest GSH levels compared the other groups (p<0.001). GSH levels of group 6 was lower than group 5, however, higher the other groups (p<0.001). Group 2, 3 and 4 GSH values were lower groups 5 and 6, but higher than groups 1, 7 and 8 (p<0.001).The highest GPx levels were determined in group 5 (p<0.001). Group 2 GPx value did not different from group 6, however, it was significantly higher than groups 1, 3, 4, 7 and 8 (p<0.001. This parameter in group 6 compared to group 3, but higher than groups 1, 4, 7 and 8 (p<0.001).Group 5 has the highest SOD levels (p<0.001). SOD levels in group 7 were lower tha group 5, higher than other groups (p<0.001). Group 2 SOD values were not different from group 6, lower than group 5 and singificantly higher than the other groups (p<0.001). Groups 3 and 4 SOD parameter were higher than grup 1, 7 and 8, however, it was lower than groups 2, 5 and 6 (p<0.001).Plasma lactate levels in group 7 were higher than the other groups (p<0.001). This parameter in group 4 lower than group 7 and higher than other groups (p<0.001). Group 1 and 6 lactate levels were lower than groups 4 and 7, but higher than groups 2, 3, 5 and 8 (p<0.001). Group 5 lactate values were higher than groups 3 and 8, lower than the other groups (p<0.001).The highest serum zinc levels were determined in group 2 (p<0.001). Group 5 serum zinc parameter was lower than group 2 and higher than other groups (p<0.001). It was determined that Group 1 zinc levels were lower than groups 2 and 5, higher than other groups (p<0.001). Group 3 and 6 zinc levels were higher than groups 4, 7 and 8, but much lower than group 1, 2 and 5 (p<0.001). Zinc levels in group 4 were higher than groups 7 and 8, however, lower than other groups (p<0.001).The results of present study showed that zinc supplementation prevents increased free radical production, inhibition in antioxidant activity and muscle fatigue in diabetes and acute exercise. In diabetes and acute exercise, zinc supplementation in physiological doses may be useful in terms of health and performence