The effects of pterostilbene and resveratrol on the biomechanic, biochemical and histological parameters in streptozotocin-induced diabetic rats gastrocnemius muscle

It was observed that adverse effects of diabetes on skeletal muscles such as changed muscle contractility and decreased in contractile force. Several studies have been shown that the applications of antioxidant resveratrol and pterostilbene enhanced the metabolic parameters in diabetic rats. The aim of this study is investigation of the effects of pterostilbene (PTS) (trans-3,5-dimethoxy-4-hydroxystilbene) and resveratrol (RSV) (trans-3,5,4'-trihydroxystilbene) applied at different doses in the treatment of streptozotocin-induced diabetic myopathy. In our study were used 80 rats of Wistar albino species ranging in weight from 250-300g. Rats, Control (n=10), Diabetes (n=10), Diabetes+10mg/kg PTS (n=10), Diabetes+20mg/kg PTS (n=10), Diabetes+40mg/kg PTS (n=10), Diabetes+10mg/kg RSV (n=10), Diabetes+20mg/kg RSV Diabetes+(10+10) mg/kg PTS/RSV (n=10) were divided into 8 groups. Streptozotocin (STZ) was injected from the tail vein of rats 45 mg/kg/dl in diabetic groups. At the end of 5-week experiment period, gastrocnemius muscle preparations were removed quickly and biomechanical recordings were taken. Then it was recorded electron microscopy images of tissues taken from the rat gastrocnemius muscle preparations. Blood glucose, serum insulin and malondialdehyde levels were analyzed taken blood from rats. As a result, PTS applications have increased more than RSV applications that the skeletal muscle isometric contraction forces decreased with diabetes. In addition, blood glucose, serum insulin and malondialdehyde levels were more normalized in PTS applications. When the electron microscopic images of diabetic rats treated with antioxidants were examined, the healing effect of the Mix group in Type-1 DM was relatively better than in the other groups.