The effect of Stromal Vascular Fraction on wound healing and scar maturation: Experimentral study on rabbits

Scar maturation is fewest known phase of wound healing. Usually scar tissue complete this period normally but some of them hypertrophied. Medical or surgical many treatment protocols have been reported, but exact treatment has not been defined yet. By this study we planned to find the effect of fat graft and stromal vascular fraction (SVF) on wound healing and scar maturation.So we decide to use the most common hypertrophic scar animal model reported in the literature. Fourty wounds were made at 20 ears of 12 rabbits. Two wounds, each wound was 7 mm diameter, were made at left ear of group 1 (control group) rabbits and 2 wound for both of the ears of group 2 (fat graft group) and 3 (SVF group). The effects of fat graft and SVF on scar maturation were compared with control group. Dermal cellularity and scar elevation index (SEI) had quantitatively; vascularity, inflammation, MMP-2 and TGF-ß1 antibody staining had semi-quantitatively evaluated.After fat graft and SVF application, the quantity of dermal cellularity, vascularization, scar elevation index had increased. But statistically, this increase is not significant. TGF-ß1 antibody staining was minimally better than MMP-2 antibody staining. We thought that this increase of inflamation, dermal cellularity and SEI was according to TGF-ß1. Collagen alignment was better at study groups than control group.The result of the study is fat graft and SVF contribute to dermal cellularity, increase dermal thickness and collagen volume. Fat graft and SVF contribute to reorganize and correct the alignment of collagen synthesized from fibroblasts, but the main disadvantage is increase at dermal thickness and collagen volume .