Study of some molecular genetic properties of thalassemia a (ALFA)

Thalassemia, which is an inherited disease that is generally seen in races belonging to Mediterranean countries, occurs as a result of a defect in hemoglobin production. Inherited disorders in the alpha and beta chains, two subunits of the globin part, in hemoglobin cause thalassemia. In our study, 120 of the patients who applied to the Hereditary Blood Diseases Center in Tikar Province of Iraq were reached and the experimental group was formed. As a result of the experimental study performed using samples taken from the patients, significant results were obtained regarding the molecular diagnosis of thalesemia. In this study, genetic molecular analysis of thalassemia was carried out. SYBR-PCR method was preferred as a fast and reliable method for deletion alleles and the results were compared with classical PCR method. Band appearance could not be obtained from the PCR products obtained from agarose gel electrophoresis, but approximately 20 times more efficient results were obtained in the SYBR-PCR method. Finally, 120 DNA samples were tested with 4 different SYBR PCR / DC analysis techniques. The results were found to be consistent with the mPCR / gel electrophoresis method and more sensitive. KEYWORDS: Thalesemia, Molecular Diagnosis, Genetic Features, PCR, DNA