The effects of %20 IV mannitol in experimental models of subarachnoid hemorrhage (SAH) in rabbits

Objective: This study aimed to examine the effects of mannitol , an osmotic diuretic agent in experimental models of subarachnoid hemorrhage (SAH) in rabbits.Mannitol is used frequently in the event of increased intracranial pressure on the intravenous administration of mannitol 20% of cerebral vasospasm . Material and Method: In this study a total of 28 New Zealand white rabbits were used. Rabbit Group 1 [control, n = 7] and Group 2 [SAH (+), n = 7] Group 3 [SAH (-) 20% Mannitol (+), n = 7] Group 4 [SAH (+) 20% Mannitol (+), n = 7] were divided into 4 groups. SAH has created one of the rabbits in groups 2 and 4 cisterns manga given the autologous arterial blood. Group 3 and 4 rabbits were 1g/kg/day intravenously for 3 days, 20% mannitol in 4 equally divided doses. All rabbits were sacrificed 72th h after the formation of SAH in the brain and brain stem were removed globally and fixed. The subjects of the basilar artery sections obtained from stained with hematoxylin and Eosin were examined under a microscope. Results: The resulting thickness of basilar artery cross-sectional vessel, the vessel diameter, lumen diameter and lumen cross sectional area and total vessel area was measured. There was no significant difference between the 1,3,4. groups in terms of vessel wall thickness(p=0,01). Among all groups, maximum vessel wall thickness was measured in the group 2. Compared to Group 1 and 3, there was no significant difference between the basilar artery lumen diameter(p=0,46). There was no significant difference between the basilar artery lumen cross-sectional area(p=0,08). 20% mannitol therapy on developing experimental models of cerebral vasospasm after subarachnoid hemorrhage has been found to have any beneficial or adverse effects. Keywords: subarachnoid hemorrhage, serebral vasospasm,%20 mannitol, basillar artery, experimental SAH model