Against apoptotic effect of cisplatin on testis protective effect of thymokinone

With this thesis, we aimed to eliminate or minimize the adverse effects of Cisplatin, an antitumor drug, Cisplatin, on the testicular tissue with thymoquinone, which is known to have biological effects and has increased in recent years. For this purpose, 29 Wistar albino rats randomly 1. Control (n = 4), 2. Sham (n = 4), 3. Cisplatin (n = 7), 4. Cisplatin + (n = 7), 5. Thymoquinone (n = 7) was divided into groups. Corn oil (500 μl ) was administered to the second group of Sham group by gavage for 10 days. 3. Cisplatin group 1.5 mg / kg / day cisplatin 3, 5 and 7. days were administered by intraperitoneal injection. 4. Thymoquinone + Cisplatin group; corn oil (500 µl) by gavaj + thymoquinone 50 mg / kg / day and cisplatin 3, 5 and 7. on days, 1.5 mg / kg / day was administered intraperitoneally. The 5th group of Timokinon group was given thymocinone 50 mg / kg / day + corn oil (500 μl) by gavage for 10 days. In the first stage of the experiment, the body weights of the rats were measured. On the 10th day of the experiment, testes of the subjects were taken under anesthesia, and the testicles taken for histopathological examinations were routinely processed. Sperm counts were performed from samples taken from the epididymis. Sperms were stained with hematoxylin eosin. The testicles were stained with HE, Gomori trichrome and PAS paints. Immuno histochemical staining was performed with Cleaved Caspase - 3 antibody. As a result of light microscopic examination; It was found that thymoquinone reduced testicular damage and germ cell apoptosis caused by cisplatin.