Molecular identification of arabis mosaic virus, grapevine fleck virus and grapevine syrah virus-1 in grapevine fields in tokat province

With this study carried out in the province of Tokat, the presence of some viral factors causing yield loss was investigated in Merkez, Erbaa, Niksar and Pazar districts where vineyard cultivation is intense. Collected 196 grapevine samples were subjected to RT-PCR, a molecular method, using virus-specific primers to detect the presence of Grapevine fleck virus (GFkV), Grapevine Syrah virus-1 (GSyV-1) and Arabis mosaic virus (ArMV). As a result of testing the grapevine samples taken from Tokat province by RT-PCR method, expected levels of DNA amplifications were observed for ArMV (429 bp), GFkV (714 bp), GSyV-1 (611bp). As a result of RT-PCR analyses, 18 samples ArMV (9.2%), 13 samples GSyV-1 (6.6%), 1 samples GFkV (0.5%) were determined respectively. ArMV was mostly found in Erbaa district, GSyV-1 was mostly found in Pazar district, and GFkV was found only in central district of Tokat province.