Investigation of apoptotic effects of thoracic aortic pulsed magnetic field with contraction parameters

Programmed cell death (apoptosis) is the depletion of harmful, aged and unwanted cells by the organism in a programmed manner. Cell death should be regular and controlled because it causes a number of diseases such as cell death, autoimmune diseases, neurodegenerative diseases, ischemic diseases, insulin dependent type diabetes, hepatitis C infection and AIDS. In studies to understand the effects of magnetic field forces at different intensities and frequencies affecting biological systems, apoptosis has been shown to influence the proliferation of the pulsed magnetic field (PMA), the synthesis and release of growth factors. In this thesis study, it is aimed to investigate the possible apoptotic effects of PMA applied to experimental animals (rat) in the vascular system via intracellular Ca2+ concentration change and smooth muscle contraction responses. The first group of two experimental animals was exposed to PMA (1.5 mT and 40 Hz) for a one hour at the same in each day. The second group was divided into control groups. The tissues from both groups were suspended in endothelial and endothelial organ system. The tissues were treated with electrical stimulus KCl, chemical stimulant phenylephrine and H2O2 (acute and short term, 40 min incubation). The effect of apoptotic changes on the contraction of the cell at the molecular level was checked by measuring the contractile responses. In addition, the cell culture cells obtained from rat aortic aorta tissues were loaded with Fura-2am fluorescence dye and the concentration of Ca2+ in the fluorescence microscope was observed. The effects of PMA were tested with an apoptosis-inducing H2O2 to find that the Ca2+ level was higher in the PMA group. The% contraction values for both KCl and phenylephrine were found to be greater for endothelials. Phenylephrine contraction responses in tissues subjected to early apoptosis after a 40-minute incubation of H2O2 also increased in the PMA group. Again, in the tissues with 40 minutes of early apoptosis with H2O2, the KCI stimulus was found to be more susceptible to endothelial activity for the control groups. Inositol 1,4,5-triphosphate receptors, which form part of the phenylephrine response, are thought to be apoptosis critical regulators and may be an important target in drug studies.