The investigation of the effect of nigella sativa oil on oxidative/nitrosative stress in the salivary gland of rats exposed to total cranial irradiation

This study is an experimental study conducted to determine the protective effects of black seed oil in the prevention of harmful effects of free radicals generated by radiotherapy in rats. In the study, 36 male Wistar-Albino rats weighing 200 ± 20 grams were used. In the study, 2 control groups and 2 experimental groups were formed. In the study, nitric oxide synthase (NOS), nitric oxide (NO•), peroxynitrite (ONOO--), malondialdehyde (MDA) and xanthine oxidase (XO) values were investigated in each group. The values obtained from the study were analyzed with SPSS for Windows version 22 package program. As a result of the statistical analyses it has been determined that NOS, NO, peroxynitrite, malondialdehyde (MDA) and xanthine oxidase (XO) values were found to be significantly higher in ionized radiation group than all the groups. On the other hand NOS and MDA values were higher in the control group given black seed oil than the other groups, if the ONOO-- and MDA were higher, the differece between the groups was not significant. Considering these results, it can be said that the oil of black seed oil is antioxidantly effective as radioprotective agent. Key words: Radiation, Radiotherapy, Black seed, Oxidative stress, Nitrozatif stress