Phytochemical investigation of Cirsium italicum (Savi) DC. (Asteraceae) plant wich growing in Trakya region and determination of its antimicrobial and antifungal activity

In this study, Cirsium italicum (Savi) DC. (Asteraceae), which grows in Trakya region. Phytochemical examination, antimicrobial and antifungal activity determinations in the crude extracts isolation of secondary metabolites and determination of molecular structures. C. italicum plant, in the scope of the study was collected from Trakya region on July 2017. After the C. italicum plant was dried, it was extracted with methanol by maceration, and the crude extract was obtained after the methanol was evaporated. The crude extract was then back-extracted with n-hexane, dichloromethane, ethylacetate and n-butanol according to the polarity order. Antimicrobial and antifungal activity was investigated for the four extracts obtained. According to the results of antimicrobial and antifungal activity in crude extracts, the highest activity of extract was found to be ethylacetate and ethyl acetate extract was selected for isolation study. In addition to the ethylacetate extract, the crystals formed when the methanol used for the maceration process was evaporated were collected and this crystallized isolation study was applied. Fractions obtained therefrom were checked by thin layer chromatography (TLC) and similar fractions were combined. Obtained lower fractions; column chromatography (CC), TLC and reverse phase column preparative HPLC. The chemical structures of the isolated pure substances were determined using 1D and 2D NMR (APT, 1H-1H COZY, HMBC, HSQC) and mass spectroscopy (MS) methods. As a result of this study, 7 compounds were isolated, 2 phenolic acids, 2 isoflavonoids, 2 biflavonoids and 1 flavonoid from C. italicum plant. The isolated compounds were first isolated from C. italicum plant. Keywords: Cirsium, Cirsium italicum, flavonoid, antimicrobial activity, antifungal activity, isoflavonoid, biflavonoid.