DNA profiling in blood, buccal swabs and hair follicles of patients transplantation

Introduction and Object: Peripheral blood DNA profiles are often used in forensic identification and paternity/ kinship investigations. Studies were showed that, patients undergoing allo PBSCT, and liver transplantation, chimerism developed in blood and some tissues and this chimerism was important to show posttransplant engrafment and to diagnose some of complications. Nowadays, due to the increasing number of patients who had undergone transplantation, questioning the story of transplantation has gained importance in forensic cases and buccal swab or hair follicles samples was raised rather than peripheral blood samples to get more accurate results in such cases. In this study, it is aimed to investigate availability of chimerism analysis of blood, hair follicles and buccal swab samples in forensic identification of stem cell and organ transplant patients.Materials and Methods: Blood, hair follicle and buccal swab samples of five patients who were made alloPBSCT by Department of Hematology DEU and 35 patients who were made liver transplantation by Department of General Surgery DEU were analyzed as blind. After the isolation of samples DNA, specific short tandem repeats sequences were amplificated with PCR and then genotype was determined with capillary electrophoresis.Results: DNA profiles were compared with patients who underwent allo-PBSCT in four blood samples, hair and buccal swab samples were completely different, there was no mixed chimerism. It was learned that one patient whose three examples were same had failure of engraftment and transmission repetition scheduled.Discussion and Conclusion: It is showed that after succesful allo PBSCT, DNA profiles of peripheral blood samples show donor chimerism, DNA profiles of buccal swab samples varies with time after transplantation, and DNA profiles of hair samples are fully recepient?s. There was no difference between each of the three samples DNA profiles of 35 liver transplant patients. This situation is connected to the absence of development of GVHD.