Determination of genetic polymorphism among some pomegranate (Punica granatum L.) genotypes by TRNL-F region analysis

In our study, genetic relationships of 24 pomegranate genotypes was investigated by TrnL-f region analysis. In the study, which included 18 local genotypes from the Mardin region, there were also the Siirt-Çekirdeksiz genotype belonging to the Siirt region and five registered pomegranate varieties (Zivzik, Esin, Yılmaz, Hicaz and Onur). Genetic characterization of pomegranate genotypes and cultivars were determined using the TrnL-F region of the chloroplast genome. In pomegranate genotypes, DNA regions were amplified by using the primer of TrnL-F region and DNA sequence analyzes were performed according to PCR results. Phylogenetic trees were drawn to determine the kinship relationships of the studied pomegranate genotypes. Similar results were obtained in two dendrograms created using the Maximum Likelihood method and the Neighbor-Joining method. Sample 15 is externally attached to the subgroups formed in both dendograms and it is seen that it is the genotype that is the most distantly related to other genotypes. However, while Maximum Likelihood analysis reveals the close relationship of genotypes to each other more clearly, in the dendogram obtained as a result of Neighbor-Joining analysis, it is seen that all the others, except for sample 15, form a single group.