Researches on the activities of some plants used for wound healing in Turkish folk medicine

In the ethnobotanical studies, several plants were reported to be used for wound healing in Turkish folk medicine. Among the frequently used-plants for wound healing in folk medicine Cichorium intybus, Cistus creticus, Daphne oleoides subsp. kurdica, Daphne oleoides subsp. oleoides, Helichrysum graveolens and Viscum album subsp. album were selected as the plant materials of this study. Cichorium intybus, Daphne oleoides subsp. kurdica, Daphne oleoides subsp. oleoides and Helichrysum graveolens were found to be significantly active in the pre-screening tests. Our researches proceeded by using ?Biological Activity Guided Fractionation and Isolation Assays? on D. oleoides subsp. kurdica ve D. oleoides subsp. oleoides of which both have high wound healing activity. Ethyl acetate subextracts prepared from the methanolic extracts of D. oleoides subsp. kurdica and D. oleoides subsp. oleoides were shown to possess the highest wound healing and anti-inflammatory activities in the in vivo assays. Therefore, the ethyl acetate fractions were subjected to silicagel column chromatography to give subfarctions which were also applied on the same experimental models in order to be tested biologically. From the active subfractions Daphnetin, Demethyldaphnoretin-7-O-glucoside, Luteolin-7-O-glucoside, Triumbellin and Quercetin-3-O-glucoside were isolated by using RP-HPLC method. The flavonoid type compounds, Luteolin-7-O-glucoside and Quercetin-3-O-glucoside, which were found to be active in in vivo models, were also tested in in vitro enzymatic assays in order to elucidate the mechanism of action. Both compounds were found to possess hyaluronidase and collagenase enzyme inhibitory activities which could contribute the wound healing effect.