Determination of phylogenetic structures of some honey bee (Apis mellifera L.) populations in van province by molecular technics

Within the scope of this study honey bee specimens that collected from Van province Bahçesaray and Çatak districts have been studied to determine the phylogenetic structures, comparing MtDNA, COI, 16s rRNA, and ND2 loci with the samples in gene data banks. The collected samples were taken from the 6 domestic beekeepers and 6 migrating beekeepers. The collected samples were brought to the Molecular Genetics laboratory under suitable conditions. DNA isolation was performed from samples taken from each of 12 operators for COI and 16s rRNA of mtDNA. After genomic DNA isolation and PCR analysis, PCR yields were sent to sequence analysis. The outcoming sequences were combined using gene analysis software, DNA Dynamo Program and phylogenetic analysis was performed using MEGA 7 Program. As a result of the analyzes, it was concluded that both immigrant and indigenous bee races grown in Van province were the same. The phylogenetic tree obtained by sequence analysis of the PCR products concluded that the samples were the similar for both loci. In comparison with the existing Apis mellifera meda sequences for COI and 16s rRNA loci obtained from the data bank, these sequence with bees in this study were determined to form different branches and did not display similarity parts of them. The situation was similar for the ND2 locus, races are concentrated as Apis mellifera anatoliaca, Apis mellifera ligustica.