Stem cell and niche effect after freezing-thawing process on damaged sperm in experimental diabetic model with high glucose media

Aim: Sperm freezing is the process of freezing sperm with cryoprotectants at a temperature of -80 -196 ° C and storing them for reuse when needed. Diabetes Mellitus (DM) is insulin hormone deficiency or ineffectiveness. All cells in DM patients are under oxidative stress due to high blood sugar levels and sperm quality decreases due to cell damage caused by DM. Stem cells are precursor cells that are easily obtained and directed by factors within the body of the organism. In studies, it is known that stem cells affect other cells positively. In our study, the deficiency in the literature will be eliminated by evaluating the negative effect of stem cell and its environment on the negative effect of cryopreservation and the negative effect of diabetes. Materials and Methods: In our study, the mesenchymal stem cell was prepared as autologous, by the method of mechanical dissociation of the sperm animal's epididymis, and groups such as diabetes mimicry group and normal glucose were prepared with the experimental groups stem cell, niche and stem cell + niche. Results: In all experimental groups, cryopreservation and time increased apoptatic effect and oxidative stress, these negative effects increased much more in diabetes mimicking groups, but there was an improvement in the analysis of oxidative stress ros levels and vitality movement values in stem cell niche and stem cell + niche groups (p <0, 01) was observed. Conclusion: In the evaluation made with the results of our study; It was observed that freezing thawing increased sperm damage, Diabetes increased these damages even more, and Mesenchymal stem cell and niche applications reduced these damages.