The investigation of effect's hi̇gh fat di̇et on carbonic anhyrdrase activity in liver

Carbonic anhydrases (CA, E.C.4.2.1.1, carbonate hydrolase), which has a wide range from prokaryotes to eukaryotes, are a metalloenzyme containing Zn2+ as a prosthetic group in active site, catalyzes reversibly the dehydration or hydration of bicarbonate (HCO3-). These enzymes are involved in many physiological processes such as the regulation of intra and extracellular CO2, H+ and HCO3- concentrations, electrolyte secretion in tissues, bone resorption, tumorigenesis and urinary acidification. In this thesis study, it is aimed to investigate of the effect's high fat diet on the activity of CA, synthesized HCO3- that is the precursor of de novo lipid synthesis, in mice liver. Within the study, 16 male C57BL / 6J mice fed with high fat and standard mouse feed from Research Diets. At the end of the four-month feeding period, mice were weighed and sacrificed by decapitation. After the liver tissues were homogenized, CA activities were measured by potentiometric method. The activity of the standard diet fed mice was measured as 0.87±0.26 U/mg protein. For the rats in the high fat dietary group, the activity was 0.60±0.15 U/mg protein. The result of statistical analysis, this difference was found to be statistically significant (p= 0.038). As a result, it was concluded that the use of high-fat diet reduced activity of CA synthesized the precursor substance for de novo lipid synthesis in mice live and metabolic and hormonal changes may be caused this decrease. Keywords: Carbonic Anhydrase, Liver, Mouse, High Fat Diet