Contributions of kefir training on adipokines in wages with a HIGH-fat DIET

Obesity is one of the most important and widespread health problems of the 21st century. The most important cause of obesity is a sedentary lifestyle and unhealthy diet. Kefir is a probiotic that is thought to be effective in combating obesity. In this study, we aimed to investigate the possible therapeutic effect of kefir and contribute to the literature on this subject by examining the effects of kefir milk on lipid profile and adipokines in a mouse model fed a high-fat diet (HFD). In this study, the effects of kefir on body weight, epididymal fat weight, lipid profile (Total cholesterol, HDL cholesterol, LDL cholesterol, Triglyceride) and adipokine hormones (leptin, adiponectin, vaspin, resistin) and Irisin/FNDC5 were investigated in mice fed HFD. BALB/c strain male mice; They were divided into 3 groups as control group (n=10), HFD (n=10) and HFD+Kefir (n=10). Standard pellet feed was given to the control group,diet with 60% fat content to the HFD group, and 15ml/kg kefir was given to the other group by oral gavage in addition to the high-fat diet for 8 weeks. Lipid profile was measured on a Beckman Coulter AU5800 autoanalyzer using commercial reagents. Adipokine hormones and Irisin/FNDC5 levels were measured by enzyme-linked immunosorbent assay (ELISA) method using commercial reagents. As a result of the experiment, no significant difference was found between the body weight gains of the groups. A significant difference was found between the epididymal fat weights of the groups. Epididymal fat weights of HFD and HFD+Kefir groups were found to be statistically significantly higher than the control group. There was no significant difference between the epididymal fat weights of the HFD and HFD+Kefir groups. Total cholesterol, HDL-C and LDL-C values of HFD and HFD+Kefir groups were found to be statistically significantly higher than the control group. There was no statistically significant difference between the HFD+Kefir group and the HFD group in terms of total cholesterol, HDL-C, and LDL-C values. There was no difference between the groups in triglyceride values. Adiponectin and Irisin/FNDC5 values of the HFD+Kefir group were found to be statistically significantly lower than the control group and the HFD group. There was no statistically significant difference between the control group and the HFD group for Adiponectin and Irisin/FNDC5 values . There was no significant difference between the groups in Vaspin, Leptin, Resistin values .