Effects of commonly used fungicides in crop protection rainbow trout (Oncorhynchus mykiss) spermatozoa

In this study, the effects of captan (CPL), mancozeb (MCZ) and azoxystrobin (AZS) fungicides on rainbow trout (Oncorhynchus mykiss) spermatozoa were investigated in vitro. With this aim, spermatozoa samples were incubated with different concentrations of these fungicides for 2 hours, and then motility parameters, lipid peroxidation levels, total antioxidant capacity (TAC), superoxide dismutase (SOD) activities, DPPH inhibitions and fatty acids profiles of spermatozoa samples which are at 50% motility due to the fungicides were determined. Accordingly, ≥2 µg/L of CPL, ≥1 µg/L of MCZ and ≥5 µg/L of AZS caused significantly decreases of spermatozoa motility parameters (P<0,05). At the similar concentrations, decreases in TAK levels, SOD activities and DPPH inhibitions, and increases in lipid peroxidation levels were recorded. CPL at concentration of 10 µg/L, MCZ at concentration of 5 µg/L and AZS at concentration of 200 µg/L reduced spermatozoa motility to around %50 in the relevant samples. When fatty acids profiles of the spermatozoa samples exposure to these concentrations of the fungicides were evaluated, almost all fatty acids profiles have shown differences. Significant reductions particularly in the amount of polyunsaturated fatty acids were observed. Consequently, the different toxic effects of these fungicides have been revealed using with rainbow trout spermatozoa.