Morfological and physiological observation on 1-MCP spreyed carnations and research on the activity of ETR1-CTR1 genes

This study was conducted to determine the effect of 1-MCP mechanizm on shelf life and morphological characteristics of carnations (Dianthus caryophyllus). Three different 1 MCP (500 nl/l, 1000 nl/l doses and control) doses were applied for 24 hours at 20°C on two commercial carnation species, `Amelia? and `Natila? after harvesting. Carnations were stored at 4°C for 21 days after applications. During this storage time at 12th hours, 24th hours, 3rd day, 6th day, 10th day, 15th day and 21st day petal samples were obtained and morphological plant characteristics were determined and compared against each other. Real-Time PCR analysis were carried out to determine expression differences of CTR1 and ETR1 genes responsible from ethylene biosynthesis during storage. The results showed that, petal samples were still remain fresh and there was no ethylene expression in petal samples after 21 days of storage. CTR1 gene expression was more than ETR1 gene expression according to Real Time PCR analysis results.