Investigation of the MDM2 gene polymorphism in patients with acute myeloid leukemia

Leukemia is malign disease which originated from bone marrow lymphatic and hematopoietic stem cells. Acute myeloid leukemia is a class of leukemia which is shown that both of phenotypic and genotypic heterogeneity. This disease is descripted over all 100 cytogenetic aberattion and vary genes mutation. MDM 2 (Murinedoubleminute 2) gen is a proto-oncogen and MDM2 gen polymorphisms which studiedsome different cancer types and this is associated with lots of cancertypes. Aim of this sutdy is research of the relationship between occuring MDM2 gen 354 A/G and (MboII) -410 T\G (SNP 309) single nucleotid polymorphisms and AML. MDM2 gen polymorphisms one of them is 354 A\G region which Adenine nucleotide convert to guanine nucleotide and the other one is -410 T/G region which Timin nucleotide convert to guanine nucleotide are determined in this study. For that reason 80 AML patients who have diagnosted AML and 20 healthy people are used in this study. DNA is isolated from AML patients and control group's blood. Both of these region are worked by RT-PCR. Data analyses of 354 d A/G polymorphism is determine that all of the people who are inclueded in this study have AA genotype. Compared of MDM2 gen 354 A/G polymorphisms between patient and control groups have not found meaningful by istatistically (p<0,005). -410 T\G region polymorphism result is found that the 19 wild type (TT) of 80 AML patients, 25 heterozygote genotype (TG) AND 36 patients are mutant type (GG) genotype. At the end of the statistical analysis of MDM2 gen -410 T\G polymorphisms has found statistically significant between two groups (p˂0,05).