Investigation of ankaferd hemostat's effect on mcf-7 breat cancer cell line by proteomic analysis

Nowadays, a large number of cancer-related research is being conducted, which is one of the most difficult and deadly diseases to treat. Thanks to one of these studies, proteomics analysis, which is one of the omic techniques, cancer can be examined at the protein level and shed light on new alternatives as well as supporting current clinical studies related to the mechanism of diagnosis, treatment and diagnosis of cancer. In this study, after the MCF-7 breast cancer cell line was treated with Ankaferd Hemostat (ABS; Ankaferd Blood Stopper®) for different periods of time, comparative proteomic studies performed on ABS untreated (control, C group) and ABS treated cells (treated, T group). Thus, the effect of ABS was examined at molecular level after being applied in vitro on MCF-7 breast cancer cell line. In this study, proteins belonging to C and T groups were separated by two-dimensional gel electrophoresis. After ABS were applied, decreasing or increasing amounts of proteins were determined by image analysis studies. As a result of comparison of C and T groups, proteins which were Quantitatively differentiated at least 2-fold were analyzed by matrix assisted laser desorption/ionization, flight time mass spectrometry (MALDI-TOF/TOF-MS) and identified by peptide sequencing studies. The identified proteins were searched with the help of databases and their functions and protein-protein interactions were determined. The biological processes and pathways that ABS has affected on MCF-7 breast cancer cell have been identified with the database of String and KEGG. The obtained proteins were associated with the studies in the literature in order to explain the anticancer activity of ABS at the proteome level. Keywords: Proteomics, Breast cancer, Two-dimensional (2D) gel electrophoresis, MALDI-TOF/TOF-MS, Ankaferd Hemostat.