Immobilization of Aspergillus niger ATCC 9642 on alginate beads and investigation of its use decolorisation of some textile dyes and olive mill waste.

,002%,/,=$7,21 2) $63(5*,//86 1ø*(5 $7&& 21ALGINATE BEADS AND INVESTIGATION OF ITS USE FORDECOLORISATION OF SOME TEXTILE DYES AND OLIVE MILLWASTE.Murat UYGUNSUMMARYIn this work the optimal conditions for the immobilization of A. niger ATCC9642 on alginate beads were determined. Optimum alginate concentration was foundto be % 3 (w/v). Optimum glucose concentration of growth medium was found % 3(w/v). 0 minute and 6,75 were optimum curing time and pH, respectively. Optimumspor/alginate concentration ratio was 1/6 (v/v).Extracellular enzymes produced by immobilized A. niger ATCC 9642 wereinvestigated. Out of the four enzymes (lipase, amilase, protease and catalase) tested,lipase was the only enzyme present with low activity.Decolorization capacity of free and immobilized A. niger ATCC 9642 againstES Blue 4BL and ES Red BWS dyes was investigated. Decolorization capacity offree and immobilized A. niger ATCC 9642 against ES Blue 4BL were % 89,6 and %94,7, respectively. Furthermore, decolorization capacity of free and immobilized A.niger ATCC 9642 against ES Red BWS were % 94,1 and % 83,9 (for 75 mg/L dyesolution).øPPRELOL]HG IXQJXV GHFRORUL]HG EOXH DQG JUHHQ HIIOXHQWV REWDLQHG IURP Dtextile factory by % 17 and % 30, respectively. No decolorization capacity againstolive oil mill waste was measured.SEM images of free and immobilized fungus were compared and differencesbetween morphologies were detected. Nevertheless, it is concluded thatdifferentiation of morphologies do not effect the decolorization capacity.