Comparison of the effects of atmospheric pressure plasma and drug therapy on breast cancer cells

In this thesis, atmospheric pressure cold plasma pen system was designed to apply to MCF-7 breast cancer cells. Plasma discharges obtained by using argon and helium gases were analyzed by optical emission spectroscopy (OES) method. In the atmospheric pressure plasma pen system, argon and helium discharges were obtained at flow rates from 1 L / min to 5 L / min using kHz (7 kV - 50 kHz and 6 kV - 4 kHz) power supplies. Helium plasma up to 5 cm in length and argon plasma up to 2 cm in length were produced. Reactive oxygen and nitrogen species such as OH, NO and NII, which are important in MCF-7 cell culture study, were determined from OES. The efficacy of plasma on the liquid medium was examined before moving to the cell working phase. Atmospheric pressure plasma was treated with liquids such as distilled water, isotonic serum for 5 min and 10 min. Physicochemical changes caused by plasma in the liquid medium after the application were examined. The effect of plasma on MCF-7 human breast cancer cells was compared with doxorubicin, a common chemotherapy drug, and its common effects were evaluated. Plasma alone, Doxorubicin only, Doxorubicin + plasma groups were compared and their results on the experimental groups after 24 hours, 48 hours and 72 hours were compared. It has been observed that plasma is more effective on breast cancer cells in plasma applications than Ar plasma, and in drug + plasma applications, plasma has been observed to help long-term efficacy of Doxorubicin drug used in breast cancer treatment.