Isolation and characterization of glutathione s-transferase and glutathione reductase in cabarla strawberry variety

Reactive oxygen species (ROS) increase in plants due to abiotic and biotic stresses. ROS interfere with intracellular substances and cell membrane to damaged plants. Plants have enzymatic and non-enzymatic antioxidant system from protect themselves against ROS. Glutathione reductase and glutathione s-transferase are two of the antioxidant enzymes. Glutathione reductase converts oxidized glutathione (GSSG) into reduced glutathione (GSH) and it provides intracellular GSH / GSSG balance. Whereas Glutathione s-transferase detoxifies intracellular toxic substances. GR contains GR1 and GR2 two classes and GST contains tau, phi, lambda, theta, zeta, beta and delta classes in plants. The sequence information of these enzymes, which are extremely important for coping with stress, has not been investigated in many plants including strawberries. In this study, GR and GST antioxidant enzyme genes were isolated and characterized from Karbala strawberry variety. For this purpose, degenerated primers were designed and partial cDNAs were amplified by PCR, 3' and 5' RACE analysis were performed to obtain full length nucleotides. According to the sequence results, the GR and GST genes were composed of 2091 and 1007 nucleotides, respectively, and were found to have an open reading frame of 557 and 287 amino acids. In addition, the isolated GR gene Pisum sativum, Medicago truncatula, Cicer arietinum and GST gene Arabidopsis thaliana, Zea mays, Prosopis juliflora genes have shown a high degree of similarity. These genes, which are characterized at molecular level, can be used for the characterization of similar genes in other species as well as for the development of strawberry varieties resistant to stress conditions.