Investıgatıon of the effects of atorvastatın on secondary kıdney ınjury ın rat ıntraabdomınal sepsıs model

Purpose of the Study: Statins, show promise in kidney injury due to sepsis which has high mortality rates. The aim of this experimental study is to investigate the effects of atorvastatin in intraabdominal sepsis model.Materials and Methods: After approval of the study by DEUTF ?Experimental Animal Research Ethics Committee?, adult male Wistar rats weighing 200 ? 250 g were randomly allocated into three groups as Sham (n=7), Sepsis (n=7) and Atorvastatin (AV, n=7). The Sham group underwent only laparotomy; cecal ligation and puncture (CLP) was performed to the Sepsis and AV groups. One ml of saline orally was given twice to the Sham group 18 and 2 hours before laparotomy; the Sepsis group received 1 ml of saline twice on the same hours before CLP; the AV group was given 10 mg/kg atorvastatin per oral, using 2,5 mg/ml atorvastatin microemulsion 18 and 2 hours before CLP. Two ml of venous blood was withdrawn from vena cava inferior for Blood Urine Nitrogen (BUN) and creatinine analysis, the left kidney was removed for histopathological examination 24 hours following CLP. The rats were sacrificed afterwards. Plasma samples were obtained by centrifuging the blood samples. The kidney tissue was investigated for mononuclear lymphocyte infiltration, red blood cell extravasations and tubular changes histopathologically.Results: The BUN and creatinine values were significantly higher in the Sepsis group compared to the other groups (p<0,05).The greatest red blood cell extravasations, mononuclear lymphocyte infiltration and tubulo-interstitial injury were observed in the Sepsis group. Despite a fewer erythrocyte extravasations, mononuclear lymphocyte infiltration and tubulo-interstitial injury in the Atorvastatin group compared to the Sepsis group the difference between them was not statistically significant (p=0,237, p=0,084, p=0,131, respectively).Conclusion: The results of this study have shown that administration of Atorvastatin provides biochemically and histopathological improvement in renal injury due to sepsis.Key words: Sepsis, kidney injury, statin, atorvastatin