Invastigation of lim15 expressi̇on as A possi̇ble marker for mei̇oti̇c di̇vi̇si̇ons i̇n azoospermi̇c males

Sperm cells proliferate by meiotic division. It is important to show that meiosis is active at the testis tissue. In order to determine whether there is spermatogenic activity in azospermic patients. More than 100 genes take a role at the DNA repair mechanism. REC A protein, one of the proteins coded by these genes, works at the recombination switch and helps for a damage free transfer of complementary chain sequence. At eukaryotes, there are two REC A like proteins that work at recombinase stage: Lim15 and Rad51. Rad 51 is expressed at both meiotic and somatic cells and has a role at DNA repair mechanism. Lim15 is a protein that has a role at DNA synthesis depended on recombination at meiosis stage. Studies conducted on eukaryotes showed that Lim15 protein is expressed only at cells specific to meiotic division and showed that Lim15 protein co-works with RAD51 protein at meiosis stage. Expression of the both proteins has a critical importance during spermatogenesis. With this project, the expression of Lim15 at testicular tissue from azospermic patients will be researched. The correlation between the expression of this gene and the spermatogenetic activity will be studied. Also, expression levels of the gene will be observed for the azoospermic patients who treated by hormone drugs, or if after the treatment is there an increase in the spermatogenic activity will be researched. At the study, testical tissue will be acquired from azospermic patients undergoing TESE operation. RNA isolation will be done from tissue samples, PCR tests will be setup using real time expression kits. In this way, the presence of the gene responsible for the meiotic division for sperm production will be investigated. Also the potential for spermatogenesis will be studied. As a result, for azospermic patients, instead of recurrent TESE operations it could be possible to make an elective TESE operation follow the spermatogenic activity. Therefore, by examining the responses to hormon replacement theraphy of the patient for Sermatogenesis, it will be possible to schedule a TESE operation accordingly.