Dna amplification of some amphibian and reptilian species from collection samples

At this study, our target was to examine some amphibian and reptilian samples which were collected from Çanakkale and surroundings and protected in a collection of Biology Department of ÇOMÜ, at molecular level. DNA isolation and amplification with PCR technique steps were done with samples of 40 species of the collection. As well as DNA sequence analysis of the venomous (Montivipera xanthina and Macrovipera lebetina) and the semi-venomous (Telescopus fallax and Malpolon monspessulanus) species of the collection were made by using some advanced molecular techniques.This study contains collection samples which were collected between 2003-2009 years from different localities. The samples were collected between 2003-2005 years, were fixed by formaldehyde and preserved in ethanol. The other samples which were collected in 2006 and later, were fixed and preserved by ethanol. Six different DNA isolation protocol were determined from literature for the formalin fixed samples becaause of negative effects of formaldehyde on the molecular studies. The protocol which gave best results, was choosen anad arranged for our samples.At this study, with the DNA isolation of samples of 40 species which includes one-third part of Turkey?s Amphibian and Reptilian Biodiversity, the infrastructure of future studies were composed. These samples, considered in this sense, were collected from different localities and this would be useful for systematic and also phylogenetic studies. The performed DNA sequence analysis of venomous and semi-venomous species of collection would be important by determining DNA inventory of these rare species.As a result, it was contributed to carry on molecular zootaxonomic studies by using less living sample with the evaluation of existing collection material at molecular level.Keywords: Turkey, DNA isolation, DNA amplification, DNA sequence analysis, Urodela, Anura, Lacertilia, Ophidia.