Purification of lipoxygenase enzym from pea
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Abstract (EN)
Lipoxygenases (EC 1.13.11.12 ) catalyse the dioxygenation of fatty acidswhich contain one or more 1(Z),5(Z)-pentadien systems, yielding chiral (E,Z)conjugated hydroperoxy fatty acids.In this study lipoxygenase has been purified from pea seeds encoded by FieldCrops Department of Ç.Ü Agricultural Faculty. For this purpose pea seedshomogenized, ultracentrifuged, fractioned with ammonium sulphate precipitation andapplied on moleculer gel chromatography separation was applied lipoxygenase waspurified 71.55 U/mg fold in pea samples and specific activity of enzyme in pea wasfound as 475.81 U/mg prot,respectively.Key Words: Lipoxygenase, Pea, Moleculer gel chromatography
Author
Oya Sarı
How to Cite
Oya Sarı (Master Thesis). Purification of lipoxygenase enzym from pea, 2006, Çukurova University.
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