Investigation of histon deacetylase enzyme inhibitory effect of a nutritional bioactive compound piperlongumine

It is aimed to better understand the effects of nutrients on the organism by investigating the nutrient-gene interactions that have an important place in the field of Nutrition and Dietetics. In recent studies, many bioactive components in various foods have been identified as Histone Deacetylase Enzyme Inhibitor (HDACi). In this thesis, HDACi effect of Piperlongumine (PL), known as a bioactive nutrient component, on HeLa and HEK293T cell lines was experimentally investigated. In order to determine the effect of PL on HDAC activity in HeLa and HEK293T cell lines, the LD50 doses planned to be applied were firstly determined by MTT and AO/PI staining methods. Protein quantification in cellular lysates prepared by the NETN lysis method was carried out by the Bradford assay. With the experimental studies carried out in this study, it was determined that HDAC enzyme activity decreased in HeLa cell lines and increased in HEK293T cell lines after 24 and 48 hours of PL applied. When lysates prepared from HeLa and HEK293T cells grown under normal conditions were treated directly with PL just before HDAC activity assay, it was determined that HDAC enzyme activity increased statistically significantly in HeLa cells and decreased in HEK293T cells. Our results showed that PL has a different effect on HDAC enzyme activity in HEK293T normal and HeLa cancer cell lines. In addition, our results demonstrated that the opposite effects can also occur when HEK293T and HeLa cell lines are applied with PL for 24 to 48 hours in cell culture medium or when cellular lysates applied directly with the PL. The results obtained show that there may be a direct interaction between PL and HDAC enzymes, also PL can be effective with indirectly mechanisms on HDAC enzyme activity.