The effect of allergen molecule-reduced propolis with biotechnological transformation on apoptosis and angiogenesis in cancer cells

Propolis is a natural product where bees take benefit from its protective effects. The bees prepare the propolis from the resin-rich trees and plants by digesting the resin with saliva enzymes and mixing them with wax. Propolis protects the colony against antibacterial, antifungal and antiviral threats and prevents the bees from getting the disease. Propolis has been used for many years in the protection of human health due to its strong antioxidant, anti-inflammatory and anti-tumor effects. In addition to many benefits, certain components, especially caffeic acid esters and hydrosinamic acid esters, may cause various allergic complications. In our study, we aimed to investigate the cytotoxic, apoptic and angiogenetic effects of propolis on colon cancer and healthy colon cell lines. Propolis samples were extracted by different solvents and methods and biological transformation was performed by using different Lactobacillus plantarum strains. After transformation, content analysis of all products in LC ms / ms was made and then cell culture studies were started. The cytotoxic, apoptic and angiogenetic effects of propolis on HCT-116 colon cancer cell lines and CCD-841 CoN normal colon epithelial cell lines were investigated. All propolis samples were evaluated on 28 paramets in our experiments and the most successful samples were determined. The results of our study have reduced the allergen content by biological transformation for the first time, in many cases the amount of other polyphenols has been preserved or increased, and it has led to the emergence of an apitherapy product that protects the healthy colon cell line and a more effective cytotoxic, apoptotic, anti-polyesthetic and anti-angiogenic effect in colon cancer. Our study suggests that all propolis specimens are sensitive to the specific cell line, the best solvent to extract the polyphenol content, and recommends the use of products with safe dose range for the treatment of cancer and that this will make the propolis treatment more specific and Keywords: Propolis, Biological Transformation, Antioxidant, Apoptosis, Colon Cancer, Angiogenesis