Using mechanobiological approach methods in the analysis of living structure

Mechnanobiology is an emerging field of research that investigates the physical properties of cells and how they respond to applied forces. Cells perceive and process mechanical information transmitted by the extracellular environment to make decisions. One of the current methods used to investigate the mechanobiological aspects of cells is the use of Optical Trapping (OT) systems. Using an OT device, it is possible to trap and manipulate particles at the micron level. The trapping and manipulation processes occur as a result of the forces generated by the focused laser beam generated by a high numerical aperture (NA) objective. This method, which uses an optical tweezer system, is a powerful method for mechanobiological approaches because it is non-invasive and can be controlled with high precision. In addition, OTs are used not only in the field of mechanobiology but also in fields such as Physics, Biophysics, Biotechnology, and Materials Science. In this thesis, Saccharomyces cerevisiae (S.cerevisiae), whose complete genome sequence is known and easy to obtain, was used as the living structure examined mechanobiologically. Although there is a wide knowledge about S.cerevisiae, little is known about its mechanical properties. Within the scope of the study, the basic parameters for the trapping of S. cerevisiae using the OT system were calculated theoretically. In addition, S. cerevisiae trapping was observed experimentally using the OT measurement technique. The aim of this study is to provide a perspective on the studies in the field of mechanobiology and to provide preliminary information for future studies in this field.