Investigations of regeneration by somatic embryogenesis method in Cyclamen coum

In this thesis study, regeneration by somatic embryogenesis in C. coum was investigated using leaf and petiole explants. Various medium containing different combinations and concentrations of NAA, 2,4-D, BA, Kinetin and 2-iP were established to obtain embryogenic callus. Callus formations were observed in all experiments. Although, embryo formation was observed in medium containing NAA, BA and Kinetin, no embryo formation was observed in medium containing 2,4-D and 2-iP. The highest regeneration rates were obtained in the cultures which were established with explants from in vitro derived plantlets. In this experiment, callus formation rates were ranged from 84.21% to 91.22% in petiole explants and ranged from 87.17% to 89.74% in leaf explants. Embryo formation rates were ranged from 47.36% to 68.42% in petiole explants and ranged from 43.58% to 69.23% in leaf explants. Different stages of somatic embryos were monitorized by scanning electron microscope and differences of stages were investigated. In the study, also limited regenerations via organogenesis were observed. Plantlets obtained by somatic embryogenesis method were acclimatized successfully and 82.5% of acclimatized plants survived.