Determination of cytotoxic, genotoxic and oxidative damage in primary hepatocyte culture of deltamethrin van fish (Alburnus tarichi)

Deltamethrin is a popular and widely used pyrethroits in worldwide. Although chemical is used to combat insects, it has effects on other non-target organisms. One of these living groups is fish. Deltamethrin is extensively used in agriculture, animal husbandry and domestic areas in Lake Van basin. The aim of this study was to determine the cytotoxic, genotoxic and oxidative damage of deltametrine on primary hepatocyte culture of Van fish (Alburnus tarichi). In this study, toxic effects of different concentrations of deltamethrin in primary hepatocyte culture of Van fish were investigated by liver enzymes Aspartase aminotransferase (AST) and Alanine aminotransferase (ALT)), total antioxidant status (TAS), total oxidant status (TOS), lipid peroxidation (MDA) and DNA damage (8-OHdG). Deltamethrin caused an increase in AST and ALT levels depending on the dose and time. TAS and TOS levels increased at the end of the 24th hour and there was no difference at the end of the 48th hour. Deltamethrin did not show an effect on MDA level but increased 8-OHdG (p <0.05). In conclusion, it can be said that high doses of deltamethrin (1 and 10 µM) have toxic effect on primary hepatocyte culture of Van fish