Myricetin in experimental acute spinal cord damageevaluation of neuroprotective effects

Objective : In this project, we aimed to identify a class of flavonoid polyphenolic compounds with antioxidant properties. By applying Myricetin, which is a member of the family, to rats with traumatic spinal cord injury. Myricetin has a secondary effect on free radicals in the spinalcord after trauma. We evaluated its neuroprotective effects on injury. Method: 45 Wistar albino rats were randomized into 5 groups, 9 in each group was done. Traumatic spinal cord injury, weight reduction model with titanium rodscarried out. Group-1 (sham), rats underwent laminectomy. 7th day spinal cord sample, blood and tissue samples were taken. Group-2, rats underwent laminectomy. 0.9% saline with oral gavage (OG) done. On the 7th day, spinal cord sample, blood and tissue samples were taken. Group-3, rats underwent laminectomy. Spinal trauma was created with metal rods. With OG 0.9% saline was applied. On the 7th day, spinal cord sample, blood and tissue samples were taken. Group-4, rats underwent laminectomy. Spinal trauma was created with metal rods. with OG low dose myricetin (5mg/kg/day) was administered. 7th day spinal cord sample, blood and tissue samples were taken. Group-5 rats underwent laminectomy. Spinal trauma was created with metal rods. With OG high dose myricetin (25mg/kg/day) was administered. 7th day spinal cord sample, blood and tissue samples were taken. Basso Beattie and Bresnahan (BBB) for motor examinations of the rats on the 1st and 7th days of the experiment and Modified Tarlov tests were applied. Interleukin-1beta (IL1b) from damaged spinal cord tissue and blood obtained from rats,interleukin-10 (IL-10), total antioxidant status (TAS), total oxidative status (TOS), malonyldialdehyde (MDA) levels were determined by enzyme linked immunosorbent assay (ELISA) method our faculty Medical Biochemistry A.D. Worked in the Research Laboratory. Our Faculty Histology and Embryology A.D. spinal cord obtained from rats in the laboratorysections were examined in the histology laboratory using hematoxylin-eosin stain (H-E) examined under the light microscope. Apoptotic cells, ApopTag In Situ Apoptosis Detection Kit by TUNEL method (Millipore). TUNEL positive cells are 4 microns in gray matter. The percentages were taken by counting the sections with a 20 objective lens under the light microscope. Histopathology score and TUNEL positive cell count, one-way variance with TUKEY test statistical analysis was performed using ANOVA. Results: Myricetin caused an increase in IL-10 levels in serum and tissue independent of dose. While a decrease was observed in serum IL-1 beta levels independent of the drug dose, a significant decrease was observed in IL-1beta levels in the high-dose drug group in tissue samples. It was determined that when the application dose of myricetin increased, it created a more pronounced antioxidant response, causing a decrease in TOS level and an increase in TAS level. Again, when the application dose of Myricetin was increased, it was observed that MDA levels, which are strong markers of oxidative stress, caused a significant regression. In the histopathological examination, it was determined that TUNEL positive cell count and apoptotic index were significantly reduced by the effect of Myricetin. The lowest apoptotic index was determined in the high-dose drug group compared to the other experimental groups. Clinically, Basso-Beattie-Bresnahan (BBB) locomotor rating scale and Modified Tarloc Scale, post-traumatic Myricetin showed improvement in neurological examination. Conclusion: In the light of the biochemical, histopathological and neurological examination results we obtained, myricetin has shows anti-inflammatory, antioxidant, antiapoptotic and neuroprotective activity on secondary spinal cord injury.