Detection of Listeria spp. from ice-cream by classical culturel methods and molecular methods

Listeria spp. are widespread pathogens important for human health. They grow easily at refrigerator temperature, resistant to chilling, heating and drying process and they can exist in ice-creams more than the other pathogens.In this study a total of 60 ice-cream samples (30 plain and 30 flovoured) belong to different 4 pastry-shop (16 samples), 10 small plants (40 samples), and 1 brand (4 samples) in Çukurova region. These samples were examined for Listeria spp.Listeria suspicious colonies were isolated from 8 samples of 60 by classical culturel mthod. The Listeria suspicious colonies were determined by microbact kit for biochemical properties and PCR for molecular identification.As a result, it was observed that there were no Listeria spp in ice-cream samples. It was found that Listeria isolaton was negative by molecular method, too.Moreover, it was found that to study both classical culturel and molecular methods together for isolation and identification of Listeria spp. was more successful,for evaluation the results.