Molecular study and detection of pvl toxin in methicllin resistant staphylococcus aureus (MRSA) strains isolated from different clinical samples

Staphylococcus aureus is a toxigenic bacterium, and one of the toxins' effects is to impair antibiotic efficiency. PVL toxin is one of the bacteria's virulence factors, and it is usually seen in methicillin-resistant isolates of S. aureus. MRSA can be identified quickly. It is critical for both treatment and infection control policy implementation. The purpose of this study was to look at the frequency of the PVL gene in methicillin resistant S. aureus (MRSA) isolates from various clinical samples to investigate their antibiotic susceptibility and biofilm forming potential. To validate the diagnosis, the bacteria was recognized using culture morphology and biochemical features as well as the VITEK 2 compact system. S. aureus isolates were detected in 40 (26.6%) of 150 samples. The antibiotic susceptibility of the isolates revealed that Methicillin had the highest resistance (100%), followed by Cefepime (70%), Azithromycin (57.5%), and Cefoxitin (55%). Rifampicin and chloramphenicol resistance rates in the isolates were 0%, followed by Vancomycin (2.5%), Gentamycin (5%), and Meropenem (17.5%). The isolates have the potential to produce biofilms. The tissue culture plate approach revealed that 25%, 65%, and 10% of the S. aureus isolates were strong, moderate, and weak biofilm producers, respectively. PCR detection of PVL genes indicated that this gene was present in 85% of the isolates.