Distribution of leptin, ghrelin, obestatin and receptors in cow placenta

Aim: The purpose of this study was to determine the cell localization and possible physiological functions of Leptin, Ghrelin and Obestatin and Receptors during pregnancy in cow placenta. Materials and Methods: In our study, 44 pregnant Holstein cow uterus and placenta were used. To determine the period of pregnancy of the uterus and placenta, the forehead-rump length of the fetuses were measured and age determinations were made. Tissue samples were taken from placentomal and interplasentomal pregnant cornu, and fixed in 10% neutral formalin solution. Following routine histological procedure, the tissues were blocked in paraffin. 5 micrometer sections were taken from the prepared blocks and placed on slides. In the sections obtained, to determine leptin, ghrelin and obestatin and its receptors (Lep-R, GHS-R, GPR39) strepavidin peroxidase method was applied. Results: Localization of leptin, ghrelin, obestatin and its receptors (Lep-R, GHS-R, GPR39) from the uterine mucosa in the interplasentomal region and the feto maternal part of the placenta at different periods of pregnancy in cows, It has shown that these factors are important in placental development and maintenance of pregnancy, as in other mammalian species. İt strongly supported that leptin, ghrelin, obestatin and their receptors (Lep-R, GHS-R, GPR39) may play a physiological role in growth, differentiation, migration and regulation of survival time during placentation. Key words: Cow, Placenta, Leptin, Ghrelin, Obestatin, OBR, GHS-R, GPR39