Total phenolics and total flavonoids in ginkgo biloba leaves of the plant optimization of the extraction conditions

In recent years, due to the increased interest of consumers for natural products and artificial antioxidants, the negative effects on health, the limitation of its use to prevent oxidative degradation of natural antioxidants and as a preservative in foods obtained from plant sources research has increased. Free radicals in foods and why they are decomposition reactions outside of living cells and organ systems, carcinogens, premature aging, cardiovascular diseases as major causes of such health effects. In this direction, slow down or stop the adverse health effects that are caused by free radicals that are high in antioxidant content foods should be consumed, since the interest and demand for these products has increased. Due to the high antioxidant properties some plants has been the subject of numerous investigations in recent years Bitkisi Ginkgo biloba is one of them. In this study, the antioxidant content of the plant Ginkgo biloba were investigated. Phenolics and flavonoids that have an antioxidant effect, provides benefits and many different biochemical compounds in medicinal plants are. Natural plant leaves for the extraction of phenolic and flavonoids that can adversely affect environmental and human health from toxic chemicals used in some methods instead of short extraction time, minimum organic solvent consumption with high efficiency of ultrasonic-Assisted Extraction (UAE) method has been developed. In this study, the ethanol concentration on extraction yield, extraction parameters such as the effects of time and temperature were investigated. For optimization response surface methodology (RSM) was used. For this, experimental conditions on the extraction yield: ethanol concentrations (% 25-75 ), solid/solvent ratio (100 mg/30-70 ml sample), the extraction time (15-60 minutes), temperature (30-70 ˚C) was determined. Of the best combinations of these parameters by response surface methodology was generated. Operating conditions for Best extraction efficiency, ethanol concentration:75%, extraction time:45 minutes, temperature:50 ˚C. The optimization results obtained for the amount of phenolic material were: Extraction time: 31,220, Extraction temperature: 54,119, Ethanol concentration: 57,944. Optimization results for the amount of flavonoid substance: Extraction time: 47,883 min. Extraction temperature: 36.336 ˚C Ethanol concentration: 69.512%. In addition, antibacterial effect of Ginkgo Biloba Leaves was investigated using Disk Diffusion Test method and it was determined that it showed antibacterial properties against S. aureos bacteria. Code Of Science : Department of Chemistry Keywords : Ginkgo biloba,phenolic,flavanoid,extraction,optimization