The determination of relationship between metal content of total phenol/flavonoid amounts and antioxidant activities in linden (Tilia cordata) and mint (Mentha piperita)

In this study, determination of total phenol, flavonoid, antioxidant activity and metal content (Na, K, Ca, Mg, Fe, Zn, Mn, Cu, Ni, Cd, Pb, Co, Al) for linden and mint supplied as packed unprocessed from three different regions and processed in three different brands, the relationship between the total amount of phenolic/flavonoid and antioxidant activity with the amount of metal was aimed to investigate as statistical. The UV-VIS spectroscopy and ICP-OES technique were used in determine the amount of total phenol/flavonid and metal, respectively. The gallic acid in determination of total phenol and quercetin in determination of total flavonid were used as standard. The antioxidant activity was determined by the method of DPPH scavenging activiy. In determine the amount of total phenol/ flavonoid and metal in the mint and linden samples was applied infusing, decoction, dry ash and extraction processes. The total amount of phenolic by decoction, infusion and extraction was found the highst in the mint (14769±108 µg GA/gr sample, 19339±346 µg GA/ gr sample). The total amount of flavonoid by decoction, infusion and extraction was found the highst in the mint as well. (34327±1627 µg QE/gr sample, 54691±11828 µg QE/ gr sample and 45800±1627µg QE/gr sample). Generaly, The most amount in total phenol and flavonoid was determined that found via infusion method. The metal content according to the decoction, infusion and was observed that found higher by the dry ash process. And the antioxidant activity in mint tea and linden consumed in tea bags, was found that is less than antioxidant activity determined in the lime and mint tea unprocessed.According to the results obtained, the variation according to the metal content of the total phenol/flavonoid and antioxidant activities was evaluated statistically (SPSS 17.0).